Factsheet for PGSC0003DMT400088862

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400053153

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G48690.1	PGSC0003DMT400053153	AT5G10110.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400066386	Potato	cytosol	79.36	87.5
PGSC0003DMT400066392	Potato	cytosol	78.2	85.94
PGSC0003DMT400091080	Potato	cytosol	77.62	85.58
PGSC0003DMT400066389	Potato	cytosol	70.64	83.22
VIT_03s0063g00740.t01	Wine grape	cytosol	49.42	52.8
VIT_03s0063g00750.t01	Wine grape	cytosol	49.13	52.32
KRH50256	Soybean	cytosol	46.51	50.31
GSMUA_Achr3P06860_001	Banana	cytosol	35.47	49.8
CDX77920	Canola	cytosol	45.06	49.68
Bra025117.1-P	Field mustard	cytosol	43.31	49.01
KRH71162	Soybean	cytosol, nucleus	47.38	48.95
AT2G03550.1	Thale cress	cytosol	44.19	48.72
GSMUA_Achr9P24230_001	Banana	cytosol	37.21	48.3
Bra019558.1-P	Field mustard	cytosol	45.35	48.3
KRH71161	Soybean	cytosol	38.08	47.99
AT3G48690.1	Thale cress	cytosol	45.06	47.84
CDY37916	Canola	cytosol	44.77	47.68
PGSC0003DMT400020612	Potato	cytosol	43.31	47.0
AT1G19190.1	Thale cress	cytosol	43.31	46.86
GSMUA_Achr6P04360_001	Banana	cytosol	39.83	46.76
CDX83045	Canola	cytosol	43.9	46.6
KRH50257	Soybean	cytosol	43.9	46.46
AT1G49660.1	Thale cress	cytosol	43.02	46.4
PGSC0003DMT400081445	Potato	cytosol	43.02	46.4
Bra029913.1-P	Field mustard	cytosol	43.9	46.32
PGSC0003DMT400066385	Potato	cytosol	41.28	46.25
GSMUA_Achr6P04230_001	Banana	cytosol	31.4	46.15
CDY67024	Canola	cytosol	43.31	46.13
AT3G48700.1	Thale cress	cytosol	43.9	45.9
CDX68486	Canola	cytosol	43.02	45.82
CDY41024	Canola	peroxisome	43.02	45.82
TraesCS5D01G248500.1	Wheat	cytosol	42.15	45.74
CDY28094	Canola	cytosol	42.15	45.17
CDY20870	Canola	cytosol	41.86	45.14
CDY29339	Canola	cytosol	41.86	45.14
Bra018807.1-P	Field mustard	cytosol	42.73	45.09
Bra029912.1-P	Field mustard	cytosol	42.15	44.89
Bra040667.1-P	Field mustard	cytosol	41.57	44.83
CDY51035	Canola	cytosol	42.44	44.79
GSMUA_Achr7P18960_001	Banana	cytosol	42.15	44.75
KXG35781	Sorghum	cytosol	40.7	44.73
Zm00001d025549_P001	Maize	cytosol	41.86	44.72
TraesCS5B01G241000.1	Wheat	cytosol	41.86	44.44
Zm00001d020737_P001	Maize	extracellular	40.7	44.44
CDX96674	Canola	cytosol	41.86	44.31
CDY28098	Canola	cytosol	41.28	44.1
TraesCS7B01G077500.1	Wheat	golgi	41.28	43.96
TraesCS2D01G498000.1	Wheat	cytosol	40.99	43.93
HORVU5Hr1G069110.1	Barley	cytosol	41.28	43.83
HORVU5Hr1G069140.1	Barley	cytosol	41.28	43.83
TraesCS5A01G243000.1	Wheat	cytosol	41.28	43.83
CDY51033	Canola	cytosol	40.7	43.75
Bra025714.1-P	Field mustard	cytosol	30.23	43.7
TraesCS5A01G437300.1	Wheat	cytosol	42.15	43.67
TraesCS5D01G444200.1	Wheat	cytosol	42.15	43.67
HORVU2Hr1G110800.1	Barley	cytosol	40.99	43.65
TraesCS5A01G243100.1	Wheat	cytosol	40.99	43.52
TraesCS5D01G249600.1	Wheat	cytosol	40.99	43.52
TraesCS2D01G315300.1	Wheat	cytosol	40.7	43.48
Bra016519.1-P	Field mustard	cytosol	41.28	43.43
EER99019	Sorghum	cytosol	41.28	43.43
Zm00001d005910_P001	Maize	cytosol	41.28	43.43
PGSC0003DMT400066384	Potato	cytosol	25.87	43.41
Zm00001d037288_P001	Maize	cytosol, extracellular	39.24	43.41
PGSC0003DMT400039929	Potato	cytosol	40.41	43.3
TraesCS5B01G439900.2	Wheat	cytosol	41.86	43.11
Bra018809.1-P	Field mustard	cytosol	39.53	43.04
TraesCS2B01G526100.1	Wheat	cytosol	40.41	43.03
TraesCS7D01G173500.1	Wheat	cytosol	40.41	43.03
EER88163	Sorghum	cytosol	39.53	42.9
EER96865	Sorghum	cytosol	40.7	42.81
TraesCS7A01G172600.1	Wheat	cytosol	40.7	42.81
Zm00001d050493_P001	Maize	cytosol, extracellular	40.7	42.81
PGSC0003DMT400081449	Potato	cytosol	24.13	42.78
TraesCS5A01G450100.1	Wheat	cytosol	39.53	42.63
Os09t0461700-01	Rice	cytosol	39.24	42.32
TraesCS7D01G173400.1	Wheat	cytosol	39.83	42.28
Os09t0462200-01	Rice	cytosol, extracellular	41.28	42.26
TraesCS3D01G124000.1	Wheat	cytosol	38.37	42.17
KXG26418	Sorghum	cytosol	40.7	42.17
KXG25323	Sorghum	cytosol	40.41	42.12
Zm00001d031805_P001	Maize	cytosol	33.14	42.07
AT1G49640.1	Thale cress	cytosol	38.37	41.9
TraesCS3B01G141200.1	Wheat	cytosol	38.37	41.9
Os08t0475400-02	Rice	cytosol, plasma membrane	39.83	41.9
PGSC0003DMT400066383	Potato	cytosol	36.92	41.78
CDY02986	Canola	cytosol	42.73	41.64
KRH71160	Soybean	nucleus	47.38	41.48
PGSC0003DMT400081446	Potato	cytosol	39.53	41.46
Zm00001d020744_P001	Maize	cytosol	35.47	41.36
GSMUA_Achr7P12950_001	Banana	cytosol	38.66	41.18
TraesCS5D01G068400.1	Wheat	cytosol	37.21	41.16
EER88069	Sorghum	cytosol	39.83	41.14
Zm00001d020738_P001	Maize	plastid	39.83	41.14
Os06t0214800-01	Rice	cytosol	39.24	41.03
Zm00001d016895_P001	Maize	cytosol	39.83	41.02
TraesCS2A01G497900.1	Wheat	cytosol	38.37	40.99
GSMUA_Achr9P24240_001	Banana	cytosol	38.66	40.92
EES16153	Sorghum	cytosol	39.83	40.9
TraesCS5A01G242500.1	Wheat	plastid	40.41	40.88
PGSC0003DMT400077411	Potato	cytosol	38.95	40.85
TraesCS5B01G059800.1	Wheat	cytosol	36.92	40.84
PGSC0003DMT400018674	Potato	cytosol	38.08	40.68
Zm00001d020732_P001	Maize	plastid	36.63	40.65
PGSC0003DMT400018589	Potato	cytosol	29.65	40.32
Zm00001d044921_P001	Maize	cytosol, extracellular	38.66	40.3
Os09t0460500-00	Rice	cytosol	36.63	40.26
Zm00001d020707_P001	Maize	cytosol, extracellular	33.43	40.21
TraesCS4B01G135300.1	Wheat	cytosol	37.5	40.19
EER89413	Sorghum	cytosol	39.53	40.0
KXG21709	Sorghum	cytosol, mitochondrion, plastid	32.56	40.0
OQU81663	Sorghum	cytosol	41.28	39.89
Os09t0461900-01	Rice	cytosol, plasma membrane	37.21	39.88
TraesCS5A01G242600.1	Wheat	cytosol	37.5	39.81
Zm00001d050837_P001	Maize	cytosol	38.37	39.76
PGSC0003DMT400081451	Potato	cytosol	37.79	39.76
Os09t0460700-01	Rice	cytosol	36.05	39.74
Os09t0455500-01	Rice	cytosol	36.92	39.69
TraesCS7D01G173800.1	Wheat	golgi	36.34	39.68
TraesCS2A01G317400.1	Wheat	mitochondrion, plastid	40.7	39.55
TraesCS2D01G389600.1	Wheat	cytosol	37.21	39.51
TraesCS7A01G172500.1	Wheat	peroxisome	37.21	39.51
TraesCS7B01G077300.1	Wheat	peroxisome	37.21	39.51
TraesCS6B01G046900.1	Wheat	mitochondrion	26.16	39.47
TraesCS4B01G331200.1	Wheat	cytosol	36.92	39.44
TraesCS6B01G047900.1	Wheat	cytosol	37.21	39.38
HORVU5Hr1G017510.2	Barley	cytosol	36.63	39.38
Os02t0567800-01	Rice	cytosol	36.63	39.38
TraesCS5B01G240000.1	Wheat	cytosol	36.92	39.32
AT1G49650.1	Thale cress	mitochondrion	42.73	39.3
TraesCS6A01G033400.1	Wheat	cytosol	37.21	39.26
Os06t0214300-01	Rice	plasma membrane, plastid	36.63	39.13
TraesCS6D01G038600.1	Wheat	cytosol	36.92	39.08
HORVU6Hr1G005900.1	Barley	cytosol	36.34	38.82
TraesCS4D01G328100.1	Wheat	cytosol	36.34	38.82
Zm00001d037180_P001	Maize	cytosol, extracellular	36.63	38.77
TraesCS5A01G502600.1	Wheat	cytosol	36.05	38.75
OQU85176	Sorghum	plastid	38.37	38.71
CDY16521	Canola	mitochondrion	42.73	38.68
HORVU0Hr1G005140.1	Barley	extracellular	35.47	38.61
TraesCS5D01G249700.2	Wheat	cytosol	39.83	38.59
Bra014213.1-P	Field mustard	mitochondrion	42.44	38.42
PGSC0003DMT400081450	Potato	cytoskeleton, cytosol, nucleus	36.63	38.41
HORVU2Hr1G093140.1	Barley	cytosol	36.05	38.27
CDY32845	Canola	mitochondrion	40.7	38.25
Zm00001d020740_P001	Maize	cytosol	36.63	38.18
Os09t0460400-01	Rice	cytosol	34.88	38.1
PGSC0003DMT400094975	Potato	cytosol, extracellular, plastid	30.23	38.1
Bra018808.1-P	Field mustard	plastid	42.15	38.06
OQU89622	Sorghum	cytosol	35.17	38.05
TraesCS5D01G249100.1	Wheat	cytosol	34.3	37.94
CDY51034	Canola	plastid	41.86	37.89
TraesCS2B01G409800.1	Wheat	cytosol	36.63	37.84
HORVU0Hr1G019700.1	Barley	cytosol, mitochondrion	26.45	37.76
HORVU5Hr1G068140.1	Barley	cytosol	35.47	37.65
KXG19591	Sorghum	cytosol	35.47	37.65
TraesCS5B01G235400.1	Wheat	cytosol	35.47	37.65
CDY28095	Canola	plastid	41.57	37.63
TraesCS2B01G335600.1	Wheat	plastid	40.99	37.6
TraesCS5A01G242000.1	Wheat	cytosol	34.01	37.26
TraesCS2B01G053400.1	Wheat	cytosol	37.21	37.1
KXG35782	Sorghum	cytosol	36.34	37.09
TraesCS4A01G437700.1	Wheat	cytosol	27.03	37.05
TraesCS5A01G065200.1	Wheat	cytosol, plastid	37.79	37.04
Os09t0455900-01	Rice	cytosol, plasma membrane	34.3	36.99
TraesCS5D01G244200.1	Wheat	cytosol	34.59	36.73
Zm00001d020736_P001	Maize	endoplasmic reticulum, vacuole	36.92	36.71
TraesCS7D01G173300.1	Wheat	extracellular	38.08	36.69
TraesCS5D01G249000.1	Wheat	cytosol	33.43	36.62
TraesCS5D01G076000.1	Wheat	cytosol	37.21	36.47
Zm00001d020742_P001	Maize	cytosol	30.52	36.46
TraesCS2D01G389500.1	Wheat	cytosol	31.98	36.42
TraesCS5B01G240200.1	Wheat	endoplasmic reticulum, vacuole	36.34	36.34
TraesCS5B01G072000.1	Wheat	cytosol, mitochondrion	36.92	36.29
TraesCS5D01G248700.1	Wheat	endoplasmic reticulum, vacuole	36.34	36.23
HORVU5Hr1G069040.1	Barley	endoplasmic reticulum, vacuole	36.34	36.13
Zm00001d031804_P001	Maize	cytosol	34.3	35.98
Os09t0461500-01	Rice	golgi, mitochondrion	36.34	35.92
KXG35760	Sorghum	cytosol	33.72	35.8
TraesCS5A01G242300.1	Wheat	extracellular, golgi	36.05	35.73
Os08t0474866-00	Rice	extracellular	38.37	35.68
TraesCS5B01G240500.1	Wheat	cytosol	32.85	35.65
KXG35780	Sorghum	extracellular, vacuole	36.63	35.59
Os06t0214850-00	Rice	plasma membrane	36.34	35.51
KXG25394	Sorghum	cytosol	34.01	35.45
TraesCS5D01G248900.1	Wheat	cytosol	34.59	35.42
KXG35761	Sorghum	cytosol	33.43	35.28
Zm00001d005905_P001	Maize	endoplasmic reticulum, golgi	36.05	35.23
HORVU0Hr1G013170.1	Barley	cytosol	33.72	35.15
HORVU5Hr1G093500.4	Barley	plastid	37.79	35.04
OQU80639	Sorghum	cytosol	34.01	35.03
TraesCS5A01G242100.1	Wheat	cytosol	34.3	35.01
TraesCS4D01G130000.1	Wheat	extracellular, vacuole	37.79	34.95
TraesCS5B01G412100.1	Wheat	endoplasmic reticulum	34.01	34.93
HORVU3Hr1G022640.1	Barley	mitochondrion	36.92	34.79
Os09t0462100-01	Rice	plasma membrane	38.37	34.74
TraesCS5D01G417100.1	Wheat	endoplasmic reticulum	34.01	34.72
HORVU5Hr1G069090.1	Barley	endoplasmic reticulum, golgi	37.5	34.58
TraesCS2B01G381300.1	Wheat	vacuole	39.24	34.35
Zm00001d005909_P001	Maize	plasma membrane	36.05	34.35
Zm00001d020741_P001	Maize	golgi, mitochondrion, vacuole	37.79	34.3
TraesCS2D01G360900.1	Wheat	extracellular	39.24	34.26
Os08t0475100-01	Rice	extracellular	33.14	34.23
HORVU0Hr1G013180.1	Barley	endoplasmic reticulum, golgi, mitochondrion	37.5	34.22
KXG35783	Sorghum	extracellular, vacuole	36.05	34.16
EES15127	Sorghum	extracellular	37.21	34.13
Zm00001d031801_P001	Maize	endoplasmic reticulum	35.17	34.08
KXG35785	Sorghum	extracellular, golgi, vacuole	37.21	33.95
TraesCS5D01G249500.1	Wheat	endoplasmic reticulum, golgi	37.21	33.77
Zm00001d050479_P001	Maize	extracellular, plasma membrane, vacuole	37.21	33.77
TraesCS5B01G240400.1	Wheat	mitochondrion	33.14	33.73
TraesCS5B01G240800.1	Wheat	extracellular, golgi	36.05	33.7
TraesCS7D01G046800.1	Wheat	cytosol	35.76	33.52
TraesCS5B01G240700.1	Wheat	endoplasmic reticulum, golgi	36.63	33.51
OQU80637	Sorghum	plasma membrane	38.37	33.33
TraesCS5A01G242900.1	Wheat	extracellular, golgi	36.92	33.25
TraesCS5A01G242800.1	Wheat	extracellular, plasma membrane	35.76	33.24
EES14010	Sorghum	endoplasmic reticulum, extracellular, vacuole	34.3	33.24
TraesCS5D01G249400.1	Wheat	endoplasmic reticulum, plasma membrane	35.47	32.62
OQU89623	Sorghum	mitochondrion	36.05	32.46
HORVU2Hr1G002620.1	Barley	cytosol	38.66	32.44
TraesCS2B01G006100.1	Wheat	cytosol	38.37	32.12
TraesCS2B01G006500.1	Wheat	cytosol	38.37	32.12
TraesCS2D01G015900.1	Wheat	cytosol	38.37	32.12
TraesCSU01G240900.1	Wheat	cytosol	38.37	32.12
HORVU5Hr1G098770.1	Barley	plasma membrane	33.72	32.04
TraesCS4A01G436500.1	Wheat	cytosol	35.76	31.95
HORVU5Hr1G068800.1	Barley	plastid	35.17	31.84
TraesCS5B01G239300.1	Wheat	plastid	35.17	31.84
TraesCSU01G158400.1	Wheat	cytosol	36.34	31.57
TraesCS4A01G183300.2	Wheat	extracellular, golgi	38.08	31.49
TraesCS4A01G436300.1	Wheat	cytosol	35.76	31.46
TraesCS5A01G241200.1	Wheat	plastid	34.59	30.91
TraesCS5D01G247700.1	Wheat	plastid	34.59	30.91
HORVU0Hr1G013160.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	23.26	30.77
TraesCS4A01G438000.1	Wheat	cytosol	18.02	30.24
Os09t0460300-01	Rice	plastid	34.01	30.23
TraesCS4A01G437800.1	Wheat	cytosol	36.05	29.67
TraesCS7A01G093800.1	Wheat	cytosol, plastid	36.63	29.17
TraesCS4A01G393200.1	Wheat	cytosol	36.63	29.1
TraesCS4A01G436100.1	Wheat	cytosol	35.76	28.94
TraesCS7D01G089100.1	Wheat	cytosol	36.34	28.87
TraesCS7D01G046600.1	Wheat	cytosol	35.76	28.81
HORVU7Hr1G009170.1	Barley	cytosol	35.17	28.67
Os08t0475000-00	Rice	cytosol	28.2	28.28
TraesCS5A01G241700.1	Wheat	plastid	30.81	28.19
TraesCS5D01G248100.1	Wheat	plastid	30.81	28.12
OQU76051	Sorghum	golgi, plasma membrane, vacuole	39.53	28.04
TraesCS7D01G046900.1	Wheat	mitochondrion	36.63	27.94
TraesCS5A01G407200.1	Wheat	plastid	34.3	27.7
HORVU0Hr1G004410.1	Barley	cytosol	35.17	27.69
HORVU0Hr1G013200.3	Barley	plastid	29.65	27.27
TraesCS2A01G363400.1	Wheat	endoplasmic reticulum, extracellular, vacuole	32.27	27.27
EER97903	Sorghum	cytosol	35.17	26.71
TraesCS2B01G007100.1	Wheat	cytosol	34.59	26.62
TraesCSU01G143300.1	Wheat	cytosol	34.3	26.58
Os07t0526600-01	Rice	extracellular	35.47	26.52
Os07t0501400-00	Rice	plastid	34.3	26.34
TraesCS5B01G239700.1	Wheat	plastid	30.81	25.92
HORVU2Hr1G067270.2	Barley	cytosol	31.69	25.89
HORVU5Hr1G068960.1	Barley	cytosol	31.69	25.89
TraesCS1B01G060800.1	Wheat	cytosol	32.85	25.45
TraesCS2A01G021800.1	Wheat	cytosol	32.85	25.28
TraesCS2D01G051400.1	Wheat	cytosol	33.14	25.22
TraesCS2D01G022400.1	Wheat	cytosol	33.43	25.22
TraesCSU01G008500.1	Wheat	cytosol	33.14	25.0
HORVU2Hr1G003300.1	Barley	cytosol	33.43	24.11
Os09t0435700-00	Rice	mitochondrion	28.78	23.24
GSMUA_Achr5P24460_001	Banana	cytosol, endoplasmic reticulum, peroxisome	37.5	20.94
TraesCS3D01G500400.1	Wheat	cytosol	3.49	7.5

Protein Annotations

EnsemblPlants:PGSC0003DMT400088862	EnsemblPlantsGene:PGSC0003DMG400038433	EntrezGene:102579197	Gene3D:3.40.50.1820	GO:GO:0003674	GO:GO:0003824
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	InterPro:AB_hydrolase	InterPro:AB_hydrolase_3	InterPro:IPR029058
PANTHER:PTHR23024	PANTHER:PTHR23024:SF251	PFAM:PF07859	PGSC:PGSC0003DMG400038433	RefSeq:XP_006346460.1	SUPFAM:SSF53474
UniParc:UPI000294FFE6	UniProt:M1DGW4	MapMan:35.1	:	:	:

Description

CXE carboxylesterase [Source:PGSC_GENE;Acc:PGSC0003DMG400038433]

Coordinates

chr1:+:84940791..84941825

Molecular Weight (calculated)

38156.6 Da

IEP (calculated)

4.644

GRAVY (calculated)

-0.127

Length

344 amino acids

Sequence
(BLAST)

001: MAEIVHDFFP LMRVYKDGRI ERLAGEVFVP PESDPETGVQ IKDVEIDPQI NLSARLYLPK NVDPVQKIPL FVYFHGGGFV IESAFSPTYH KYLSLVAAEA
101: KVAIVSVNYR LAPEYPLPIA HEDSWLALKW VTSHANGDGR EPWLKDNADF NLVYLGGDSA GGNIAHHIAI RFGLEKLEGV KIDGIFLACP FFWGKDPIDG
201: EGENLLAKDF VEDLVLSGNP NSTGLDKDPI DGEGENLGAK DLFEKLWLFV NPNSSGLDDP LINPEKDPKL SGLGCEKVLM YVAGKDPLRF RGLYYKEALE
301: KSGWLGTVEV VEVKDKAHVF HLFVPEAEEA MAMLKKLASF LNQS

Best Arabidopsis Sequence Match ( AT2G03550.1 )
(BLAST)

001: MDSVIAFDRS PMFRVYKSGR IERLLGETTV PPSLTPQNGV VSKDIIHSPE KNLSLRIYLP EKVTVKKLPI LIYFHGGGFI IETAFSPPYH TFLTSAVAAA
101: NCLAISVNYR RAPEFPVPIP YEDSWDSLKW VLTHITGTGP ETWINKHGDF GKVFLAGDSA GGNISHHLTM RAKKEKLCDS LISGIILIHP YFWSKTPIDE
201: FEVRDVGKTK GVEGSWRVAS PNSKQGVDDP WLNVVGSDPS GLGCGRVLVM VAGDDLFVRQ GWCYAEKLKK SGWEGEVEVM ETKNEGHVFH LKNPNSDNAR
301: QVVKKLEEFI NK

Arabidopsis Description

CXE7Probable carboxylesterase 7 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZQ91]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.