Subcellular Localization
min:
: max
Winner_takes_all: plastid
Predictor Summary:
Predictor Summary:
- cytosol 2
- mitochondrion 2
- plastid 2
- plasma membrane 1
Predictors | GFP | MS/MS | Papers |
---|---|---|---|
PPI
Inferred distinct locusB in Crop
Inferred from Arabidopsis experimental PPI
Homology
Paralog
locus | Identity | Homology Identity |
---|
Ortholog
locus | Homology Species | Location | Identity | Homology Identity |
---|---|---|---|---|
KXG22761 | Sorghum | plastid | 99.82 | 99.77 |
Zm00001d044614_P086 | Maize | plastid | 99.47 | 99.24 |
Zm00001d023218_P064 | Maize | plastid | 99.01 | 98.43 |
Os11t0104900-00 | Rice | plasma membrane | 40.02 | 97.85 |
TraesCS4A01G182300.1 | Wheat | extracellular, golgi | 97.48 | 97.14 |
TraesCS5B01G152300.1 | Wheat | unclear | 97.07 | 97.13 |
TraesCS5A01G153500.1 | Wheat | golgi, unclear | 97.07 | 97.13 |
TraesCS4D01G130900.1 | Wheat | golgi, unclear | 97.37 | 97.03 |
TraesCS5D01G158800.1 | Wheat | plastid | 96.96 | 97.01 |
Zm00001d005090_P106 | Maize | plastid | 97.95 | 96.26 |
TraesCS4B01G136100.2 | Wheat | golgi, unclear | 97.02 | 95.73 |
Os12t0104766-00 | Rice | plasma membrane | 32.18 | 95.16 |
Os11t0104866-00 | Rice | plasma membrane | 32.12 | 94.98 |
Zm00001d052308_P046 | Maize | cytosol, plastid | 96.14 | 94.37 |
HORVU4Hr1G024710.4 | Barley | plastid | 97.07 | 93.15 |
VIT_06s0004g06860.t01 | Wine grape | cytosol, plastid | 92.63 | 92.84 |
GSMUA_Achr4P16770_001 | Banana | cytosol | 92.92 | 92.38 |
GSMUA_Achr5P08520_001 | Banana | plastid | 92.22 | 92.27 |
GSMUA_AchrUn_... | Banana | plasma membrane | 73.14 | 91.98 |
HORVU5Hr1G048010.1 | Barley | plastid | 96.96 | 91.9 |
KRG97711 | Soybean | endoplasmic reticulum, nucleus | 91.4 | 91.88 |
Os12t0104800-01 | Rice | cytosol | 6.55 | 91.8 |
Solyc06g051310.2.1 | Tomato | nucleus | 91.34 | 91.77 |
VIT_13s0067g01290.t01 | Wine grape | extracellular | 91.4 | 91.67 |
KRH31163 | Soybean | endoplasmic reticulum | 91.11 | 91.59 |
GSMUA_Achr4P18860_001 | Banana | cytosol | 86.54 | 91.35 |
Solyc05g052510.2.1 | Tomato | nucleus, plastid | 91.16 | 91.32 |
KRH14933 | Soybean | nucleus | 90.58 | 91.06 |
KRH73205 | Soybean | nucleus | 90.93 | 91.04 |
CDY36318 | Canola | cytosol | 39.2 | 91.03 |
AT3G11130.1 | Thale cress | cytosol, plasma membrane | 90.46 | 90.67 |
Solyc03g096000.2.1 | Tomato | nucleus | 90.23 | 90.6 |
AT3G08530.1 | Thale cress | cytosol, plasma membrane, plastid | 90.23 | 90.55 |
KRH28591 | Soybean | endoplasmic reticulum | 90.05 | 90.42 |
KRH76885 | Soybean | endoplasmic reticulum | 89.94 | 90.31 |
CDY00997 | Canola | extracellular | 90.11 | 90.22 |
CDY00724 | Canola | cytosol | 88.65 | 90.18 |
CDY36301 | Canola | cytosol | 88.65 | 90.18 |
Bra034864.1-P | Field mustard | cytosol | 78.47 | 90.12 |
CDY07954 | Canola | cytosol | 88.24 | 90.03 |
KRH72708 | Soybean | endoplasmic reticulum, mitochondrion | 89.7 | 89.86 |
CDX73889 | Canola | cytosol | 88.47 | 89.84 |
Bra034166.1-P | Field mustard | cytosol | 75.95 | 89.64 |
CDX74011 | Canola | cytosol | 88.0 | 89.63 |
KRH17059 | Soybean | endoplasmic reticulum, nucleus | 89.23 | 89.55 |
CDY22158 | Canola | cytosol | 9.95 | 89.47 |
CDY08549 | Canola | cytosol | 80.87 | 89.45 |
GSMUA_AchrUn_... | Banana | plasma membrane | 88.65 | 88.86 |
Bra001286.1-P | Field mustard | cytosol | 76.24 | 87.8 |
GSMUA_Achr5P14240_001 | Banana | cytosol, plasma membrane, plastid | 89.53 | 87.78 |
Bra001391.1-P | Field mustard | cytosol | 73.67 | 86.71 |
CDY27997 | Canola | cytosol | 78.53 | 83.88 |
CDY35984 | Canola | cytosol | 78.23 | 83.25 |
Bra029698.1-P | Field mustard | cytosol | 71.97 | 83.16 |
CDY36317 | Canola | cytosol | 11.82 | 79.84 |
KRH12961 | Soybean | cytosol | 4.39 | 78.12 |
KRH25762 | Soybean | cytosol | 4.27 | 76.04 |
CDY22157 | Canola | cytosol | 3.45 | 75.64 |
VIT_16s0115g00130.t01 | Wine grape | cytosol | 3.98 | 58.62 |
VIT_03s0017g00210.t01 | Wine grape | cytosol, extracellular | 1.81 | 58.49 |
VIT_19s0027g01340.t01 | Wine grape | cytosol | 8.66 | 48.21 |
CDY06504 | Canola | extracellular, mitochondrion | 2.22 | 40.43 |
Protein Annotations
Gene3D:1.25.40.10 | Gene3D:1.25.40.730 | Gene3D:2.130.10.110 | MapMan:22.1.1.1 | InterPro:ARM-type_fold | UniProt:C5Y2Y8 |
InterPro:Clathrin_H-chain/VPS_repeat | InterPro:Clathrin_H-chain_N | InterPro:Clathrin_H-chain_linker_core | InterPro:Clathrin_H-chain_propeller_rpt | InterPro:Clathrin_heavy_chain | ncoils:Coil |
EnsemblPlants:EES07849 | ProteinID:EES07849 | ProteinID:EES07849.3 | GO:GO:0003674 | GO:GO:0005198 | GO:GO:0005488 |
GO:GO:0005515 | GO:GO:0005575 | GO:GO:0005622 | GO:GO:0005623 | GO:GO:0005737 | GO:GO:0005794 |
GO:GO:0005886 | GO:GO:0005905 | GO:GO:0006810 | GO:GO:0006886 | GO:GO:0008150 | GO:GO:0009987 |
GO:GO:0016020 | GO:GO:0016043 | GO:GO:0016192 | GO:GO:0030130 | GO:GO:0030132 | GO:GO:0030659 |
GO:GO:0031410 | GO:GO:0032051 | GO:GO:0048268 | GO:GO:0071439 | InterPro:IPR000547 | InterPro:IPR011990 |
InterPro:IPR016025 | PFAM:PF00637 | PFAM:PF01394 | PFAM:PF09268 | PFAM:PF13838 | PIRSF:PIRSF002290 |
PFscan:PS50236 | PANTHER:PTHR10292 | PANTHER:PTHR10292:SF1 | SMART:SM00299 | EnsemblPlantsGene:SORBI_3005G003300 | SUPFAM:SSF48371 |
SUPFAM:SSF50989 | InterPro:TPR-like_helical_dom_sf | UniParc:UPI000B4246CD | SEG:seg | : | : |
Description
hypothetical protein
Coordinates
chr5:+:274464..285813
Molecular Weight (calculated)
193550.0 Da
IEP (calculated)
5.041
GRAVY (calculated)
-0.168
Length
1709 amino acids
Sequence
(BLAST)
(BLAST)
0001: MAAANAPIAM REALTLTSLG IAPQFVTFTH VTMESEKYIC VRETSPQNSV VIIDMAMPMQ PLRRPITADS ALMNPNTRIL ALKAQIPGTT QDHLQIFNIE
0101: AKTKIKSHQM PEQVVFWKWI TPKLLGLVTQ TSVYHWSIEG DSEPTKMFDR TANLANNQII NYRCDPAEKW LVLIGIAPGA PERPQLVKGN MQLFSVDQQR
0201: SQALEAHAAS FATFKVVGNE NPSTLICFAS KTTNAGQITS KLHVIELGAQ PGKPGFSKKQ ADLFFPPDFQ DDFPVAMQVS QKYGLIYVIT KLGLLFVYDL
0301: ETAAAVYRNR ISPDPIFLTA ESSSTGGFYA INRRGQVLHA TVNDATVVPF VSGQLNNLEL AVNLAKRANL PGAENLVVQR FQELFAQTKY KEAAELAAES
0401: PQGLLRTPET VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NAFESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK
0501: VVAAFAERRE FDKILIYSKQ VGYTPDYLFL LQTILRTDPQ GAVNFALMMS QMEGGCPVDY NTITDLFLQR NMIREATAFL LDVLKPNLPE HAFLQTKVLE
0601: INLVTYPNVA DAILANGMFS HYDRPRIAQL CEKAGLYLRA LQHYSELPDI KRVIVNTHAI EPQALVEFFG TLSREWALEC MKDLLLVNLR GNLQIVVQAA
0701: KEYSEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSSSEDPD IHFKYIEAAA RTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD
0801: LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV RSLLPVEPLV DECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS
0901: NNNPEHFLTT NPFYDSRVVG KYCEKRDPTL AVVAYRRGQC DDELINVTNK NSLFKLQARY VVERMDGDLW DKVLQPENEY RRQLIDQVVS TALPESKSPE
1001: QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYVNR LDNFDGPAVG EVAVEAQLYE EAFAIFKKFN LNVQAVNVLL
1101: DNIRSIERAE EFAFRVEEDA VWSQVAKAQL REGLVSEAIE SFIRADDAAH FLDVIRAAEE ANVYDDLVKY LLMVRQKARE PKVDGELIFA YAKIDRLSDI
1201: EEFILMPNVA NLQNVGDRLY DEELYEAAKI IYAFISNWAK LAVTLVKLKQ FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IVQVDDLEEV
1301: SEYYQNRGCF NELIALMESG LGLERAHMGI FTELGVLYAR YRPEKLMEHI KLFSTRLNIP KLIRACDEQQ HWKELTYLYI QYDEFDNAAT TIMNHSPDAW
1401: DHMQFKDVCV KVANVELYYK AVHFYLQEHP DLINDMLNVL ALRLDHTRVV DIMRKAGQLH LVKPYMVAVQ SNNVSAVNEA LNELYVEEED YERLRESVDM
1501: HDNFDQIGLA QKLEKHELLE MRRIAAYIYK KAGRWKQSIA LSKKDNMYKD CMETCSQSGD RELSEDLLVY FIEQGKKECF ASCLFICYDL IRPDVALELA
1601: WMNNMLDFAF PYLLQFIREY TSKVDDLVKD KIESQKEERA KEKEEKDLVA QQNMYAQLLP LALPAPPMPG MGGPPPMGGM GMPPMGGMGM PPMGPGPMPA
1701: FGMPPMGSY
0101: AKTKIKSHQM PEQVVFWKWI TPKLLGLVTQ TSVYHWSIEG DSEPTKMFDR TANLANNQII NYRCDPAEKW LVLIGIAPGA PERPQLVKGN MQLFSVDQQR
0201: SQALEAHAAS FATFKVVGNE NPSTLICFAS KTTNAGQITS KLHVIELGAQ PGKPGFSKKQ ADLFFPPDFQ DDFPVAMQVS QKYGLIYVIT KLGLLFVYDL
0301: ETAAAVYRNR ISPDPIFLTA ESSSTGGFYA INRRGQVLHA TVNDATVVPF VSGQLNNLEL AVNLAKRANL PGAENLVVQR FQELFAQTKY KEAAELAAES
0401: PQGLLRTPET VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NAFESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK
0501: VVAAFAERRE FDKILIYSKQ VGYTPDYLFL LQTILRTDPQ GAVNFALMMS QMEGGCPVDY NTITDLFLQR NMIREATAFL LDVLKPNLPE HAFLQTKVLE
0601: INLVTYPNVA DAILANGMFS HYDRPRIAQL CEKAGLYLRA LQHYSELPDI KRVIVNTHAI EPQALVEFFG TLSREWALEC MKDLLLVNLR GNLQIVVQAA
0701: KEYSEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSSSEDPD IHFKYIEAAA RTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD
0801: LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV RSLLPVEPLV DECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS
0901: NNNPEHFLTT NPFYDSRVVG KYCEKRDPTL AVVAYRRGQC DDELINVTNK NSLFKLQARY VVERMDGDLW DKVLQPENEY RRQLIDQVVS TALPESKSPE
1001: QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYVNR LDNFDGPAVG EVAVEAQLYE EAFAIFKKFN LNVQAVNVLL
1101: DNIRSIERAE EFAFRVEEDA VWSQVAKAQL REGLVSEAIE SFIRADDAAH FLDVIRAAEE ANVYDDLVKY LLMVRQKARE PKVDGELIFA YAKIDRLSDI
1201: EEFILMPNVA NLQNVGDRLY DEELYEAAKI IYAFISNWAK LAVTLVKLKQ FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IVQVDDLEEV
1301: SEYYQNRGCF NELIALMESG LGLERAHMGI FTELGVLYAR YRPEKLMEHI KLFSTRLNIP KLIRACDEQQ HWKELTYLYI QYDEFDNAAT TIMNHSPDAW
1401: DHMQFKDVCV KVANVELYYK AVHFYLQEHP DLINDMLNVL ALRLDHTRVV DIMRKAGQLH LVKPYMVAVQ SNNVSAVNEA LNELYVEEED YERLRESVDM
1501: HDNFDQIGLA QKLEKHELLE MRRIAAYIYK KAGRWKQSIA LSKKDNMYKD CMETCSQSGD RELSEDLLVY FIEQGKKECF ASCLFICYDL IRPDVALELA
1601: WMNNMLDFAF PYLLQFIREY TSKVDDLVKD KIESQKEERA KEKEEKDLVA QQNMYAQLLP LALPAPPMPG MGGPPPMGGM GMPPMGGMGM PPMGPGPMPA
1701: FGMPPMGSY
0001: MAAANAPIIM KEVLTLPSVG IGQQFITFTN VTMESDKYIC VRETAPQNSV VIIDMNMPMQ PLRRPITADS ALMNPNSRIL ALKAQVPGTT QDHLQIFNIE
0101: AKAKLKSHQM PEQVAFWKWI TPKMLGLVTQ TSVYHWSIEG DSEPVKMFDR TANLANNQII NYKCSPNEKW LVLIGIAPGS PERPQLVKGN MQLFSVDQQR
0201: SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS KLHVIELGAQ PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL
0301: ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF ISGQLNNLEL AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES
0401: PQGILRTPDT VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NSYESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK
0501: VVAAFAERRE FDKILIYSKQ VGYTPDYMFL LQTILRTDPQ GAVNFALMMS QMEGGCPVDY NTITDLFLQR NLIREATAFL LDVLKPNLPE HAFLQTKVLE
0601: INLVTFPNVA DAILANGMFS HYDRPRVAQL CEKAGLYIQS LKHYSELPDI KRVIVNTHAI EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC
0701: KEYCEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA KTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD
0801: LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV RSLLPVEPLV AECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS
0901: NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK NSLFKLQARY VVERMDGDLW EKVLTEENEY RRQLIDQVVS TALPESKSPE
1001: QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYINR LDNFDGPAVG EVAVDAQLYE EAFAIFKKFN LNVQAVNVLL
1101: DNVRSIERAV EFAFRVEEDA VWSQVAKAQL REGLVSDAIE SFIRADDTTQ FLEVIRASED TNVYDDLVRY LLMVRQKVKE PKVDSELIYA YAKIERLGEI
1201: EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWAK LAVTLVKLQQ FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV
1301: SEYYQNRGCF NELISLMESG LGLERAHMGI FTELGVLYAR YRYEKLMEHI KLFSTRLNIP KLIRACDEQQ HWQELTYLYI QYDEFDNAAT TVMNHSPEAW
1401: EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL ALRLDHTRVV DIMRKAGHLR LIKPYMVAVQ SNNVSAVNEA LNEIYAEEED YDRLRESIDL
1501: HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD CMETASQSGD HDLAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA
1601: WINNMIDFAF PYLLQFIREY SGKVDELIKD KLEAQKEVKA KEQEEKDVMS QQNMYAQLLP LALPAPPMPG MGGGGYGPPP QMGGMPGMSG MPPMPPYGMP
1701: PMGGY
0101: AKAKLKSHQM PEQVAFWKWI TPKMLGLVTQ TSVYHWSIEG DSEPVKMFDR TANLANNQII NYKCSPNEKW LVLIGIAPGS PERPQLVKGN MQLFSVDQQR
0201: SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS KLHVIELGAQ PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL
0301: ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF ISGQLNNLEL AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES
0401: PQGILRTPDT VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NSYESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK
0501: VVAAFAERRE FDKILIYSKQ VGYTPDYMFL LQTILRTDPQ GAVNFALMMS QMEGGCPVDY NTITDLFLQR NLIREATAFL LDVLKPNLPE HAFLQTKVLE
0601: INLVTFPNVA DAILANGMFS HYDRPRVAQL CEKAGLYIQS LKHYSELPDI KRVIVNTHAI EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC
0701: KEYCEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA KTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD
0801: LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV RSLLPVEPLV AECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS
0901: NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK NSLFKLQARY VVERMDGDLW EKVLTEENEY RRQLIDQVVS TALPESKSPE
1001: QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYINR LDNFDGPAVG EVAVDAQLYE EAFAIFKKFN LNVQAVNVLL
1101: DNVRSIERAV EFAFRVEEDA VWSQVAKAQL REGLVSDAIE SFIRADDTTQ FLEVIRASED TNVYDDLVRY LLMVRQKVKE PKVDSELIYA YAKIERLGEI
1201: EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWAK LAVTLVKLQQ FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV
1301: SEYYQNRGCF NELISLMESG LGLERAHMGI FTELGVLYAR YRYEKLMEHI KLFSTRLNIP KLIRACDEQQ HWQELTYLYI QYDEFDNAAT TVMNHSPEAW
1401: EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL ALRLDHTRVV DIMRKAGHLR LIKPYMVAVQ SNNVSAVNEA LNEIYAEEED YDRLRESIDL
1501: HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD CMETASQSGD HDLAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA
1601: WINNMIDFAF PYLLQFIREY SGKVDELIKD KLEAQKEVKA KEQEEKDVMS QQNMYAQLLP LALPAPPMPG MGGGGYGPPP QMGGMPGMSG MPPMPPYGMP
1701: PMGGY
Arabidopsis Description
CHC1Clathrin heavy chain 1 [Source:UniProtKB/Swiss-Prot;Acc:Q0WNJ6]
SUBAcon: [plasma membrane,cytosol]
SUBAcon: [plasma membrane,cytosol]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.