Factsheet for PGSC0003DMT400047481

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 5
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400006608
PGSC0003DMT400010174
PGSC0003DMT400014412
PGSC0003DMT400023026
PGSC0003DMT400026656
PGSC0003DMT400047481
PGSC0003DMT400049429

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G49240.1	PGSC0003DMT400006608	AT1G72770.1	19033529
AT5G09810.1	PGSC0003DMT400026656	AT2G31200.1	21798944
AT5G09810.1	PGSC0003DMT400023026	AT2G37620.1	21798944
AT5G09810.1	PGSC0003DMT400049429	AT2G37620.1	21798944
AT5G09810.1	PGSC0003DMT400023026	AT3G12110.1	21798944
AT5G09810.1	PGSC0003DMT400049429	AT3G12110.1	21798944
AT5G09810.1	PGSC0003DMT400014412	AT3G18060.1	21798944
AT5G09810.1	PGSC0003DMT400010174	AT5G09810.1	21798944
AT5G09810.1	PGSC0003DMT400047481	AT5G09810.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc03g078400.2.1	Tomato	nucleus, plastid	100.0	100.0
PGSC0003DMT400010174	Potato	cytosol	99.47	99.47
KRH53856	Soybean	endoplasmic reticulum, mitochondrion	87.27	99.1
KRH64098	Soybean	cytosol	98.94	98.94
AT5G09810.1	Thale cress	cytosol	98.41	98.41
CDX69885	Canola	cytosol	98.41	98.41
CDY21607	Canola	cytosol	98.41	98.41
KRH43389	Soybean	cytosol	98.41	98.41
KRH59535	Soybean	cytosol	98.41	98.41
CDX85794	Canola	cytosol	92.04	98.3
CDY30296	Canola	cytosol	92.04	98.3
CDY31972	Canola	cytosol	92.04	98.3
CDY40061	Canola	cytosol	92.04	98.3
Bra028615.1-P	Field mustard	cytosol	98.14	98.14
Bra009081.1-P	Field mustard	cytosol	91.78	98.02
CDX81140	Canola	cytosol	97.88	97.88
CDY56544	Canola	cytosol	97.88	97.88
Bra006033.1-P	Field mustard	cytosol	97.61	97.61
HORVU5Hr1G117900.1	Barley	cytosol	97.61	97.61
Os11t0163100-01	Rice	nucleus, plasma membrane, plastid	97.61	97.61
TraesCS5B01G124100.1	Wheat	cytosol	97.61	97.61
TraesCS5B01G516100.1	Wheat	cytosol	97.61	97.61
TraesCS5D01G516200.1	Wheat	cytosol	97.61	97.61
KXG23031	Sorghum	cytosol	97.35	97.35
Os05t0106600-01	Rice	nucleus	97.35	97.35
GSMUA_Achr9P08850_001	Banana	cytosol	89.92	97.13
TraesCS4A01G356100.2	Wheat	cytosol	97.61	97.1
EES08028	Sorghum	cytosol	97.08	97.08
GSMUA_Achr3P02960_001	Banana	cytosol	97.08	97.08
GSMUA_Achr6P25350_001	Banana	cytosol	97.08	97.08
GSMUA_Achr8P16150_001	Banana	cytosol	97.08	97.08
KXG21029	Sorghum	cytosol	97.08	97.08
Zm00001d011086_P005	Maize	cytosol	97.08	97.08
Zm00001d035720_P002	Maize	cytosol	97.08	97.08
GSMUA_Achr9P03170_001	Banana	cytosol	96.82	97.07
GSMUA_Achr5P29190_001	Banana	cytosol	89.39	96.84
Os01t0964133-01	Rice	plasma membrane	96.82	96.82
TraesCS1B01G024500.1	Wheat	golgi, nucleus	96.82	96.82
TraesCS1D01G020000.1	Wheat	cytosol	96.82	96.82
Zm00001d053177_P004	Maize	cytosol	96.82	96.82
GSMUA_Achr11P...	Banana	cytosol	89.39	96.56
GSMUA_Achr2P16160_001	Banana	cytosol	96.29	96.29
Os03t0836000-01	Rice	plasma membrane, plastid	96.29	96.29
PGSC0003DMT400071331	Potato	cytosol	96.29	96.29
GSMUA_Achr10P...	Banana	cytosol	96.02	96.02
PGSC0003DMT400060953	Potato	cytosol	96.02	96.02
GSMUA_Achr6P34320_001	Banana	cytosol	62.07	95.9
PGSC0003DMT400076499	Potato	cytosol	49.07	95.85
EER90564	Sorghum	cytosol	95.76	95.76
GSMUA_Achr7P10410_001	Banana	cytosol	95.76	95.76
GSMUA_Achr8P02660_001	Banana	cytosol	95.76	95.76
Zm00001d019635_P001	Maize	cytosol	35.01	95.65
PGSC0003DMT400077954	Potato	cytosol	95.49	95.49
PGSC0003DMT400060225	Potato	cytosol	95.23	95.23
Zm00001d035722_P007	Maize	plasma membrane	97.08	95.06
GSMUA_Achr11P...	Banana	cytosol	94.43	94.43
Zm00001d012277_P001	Maize	cytosol	94.16	94.41
PGSC0003DMT400001169	Potato	cytosol	94.43	94.18
PGSC0003DMT400049429	Potato	cytosol	94.16	94.16
TraesCS5D01G132200.1	Wheat	golgi	92.31	93.8
PGSC0003DMT400023026	Potato	cytosol	93.63	93.63
EES01810	Sorghum	cytosol	93.37	93.62
Zm00001d042731_P002	Maize	extracellular, plasma membrane, plastid	92.84	93.09
Zm00001d016605_P001	Maize	cytosol	38.99	93.04
HORVU1Hr1G074350.1	Barley	cytosol	92.57	92.82
Os05t0438800-01	Rice	plasma membrane, plastid	92.57	92.82
TraesCS1A01G274400.1	Wheat	golgi	92.57	92.82
TraesCS1B01G283900.1	Wheat	cytosol	92.57	92.82
TraesCS1D01G274400.1	Wheat	cytosol	92.57	92.82
Zm00001d034761_P002	Maize	cytosol	95.76	92.09
AT1G49240.1	Thale cress	cytosol	92.04	92.04
CDX82326	Canola	cytosol	92.04	92.04
EES19603	Sorghum	cytosol	91.78	92.02
Bra022356.1-P	Field mustard	cytosol	78.78	91.95
CDX99315	Canola	cytosol	78.78	91.95
AT3G18780.3	Thale cress	cytosol	91.78	91.78
CDX92272	Canola	cytosol	91.78	91.78
CDX95498	Canola	cytosol	91.78	91.78
CDY18545	Canola	cytosol	91.78	91.78
CDY61982	Canola	cytosol	91.78	91.78
Zm00001d010159_P002	Maize	extracellular, plasma membrane	91.51	91.76
CDX92271	Canola	cytosol	76.66	91.17
TraesCS5A01G124300.2	Wheat	golgi	97.61	89.98
HORVU1Hr1G002840.6	Barley	plasma membrane	96.82	89.68
TraesCS1A01G020500.2	Wheat	cytosol	96.82	89.68
PGSC0003DMT400076501	Potato	cytosol	46.68	88.0
Zm00001d016508_P001	Maize	cytosol	31.57	86.23
GSMUA_Achr10P...	Banana	cytosol	62.86	86.18
KXG21030	Sorghum	cytosol	87.8	85.09
Zm00001d008746_P001	Maize	cytosol	61.8	84.73
Bra037560.1-P	Field mustard	cytoskeleton, cytosol	78.51	83.85
GSMUA_Achr2P08030_001	Banana	cytosol	77.45	83.43
Zm00001d019396_P001	Maize	cytosol	36.6	82.14
VIT_04s0044g00580.t01	Wine grape	extracellular	98.67	80.87
Zm00001d011087_P001	Maize	plastid	97.08	80.62
Zm00001d007792_P001	Maize	cytosol	55.44	79.17
PGSC0003DMT400087294	Potato	mitochondrion	56.76	78.1
HORVU5Hr1G039850.3	Barley	mitochondrion	93.37	75.7
Zm00001d003298_P001	Maize	cytosol	52.79	75.67
Zm00001d004087_P001	Maize	cytosol	41.38	72.22
Os01t0866100-01	Rice	plasma membrane	37.93	70.44
PGSC0003DMT400022210	Potato	cytosol	68.7	70.38
PGSC0003DMT400022205	Potato	cytosol	67.11	69.13
PGSC0003DMT400074882	Potato	cytosol	67.37	68.65
PGSC0003DMT400074887	Potato	cytosol	64.46	65.68
PGSC0003DMT400088088	Potato	cytosol	32.63	63.4
Zm00001d004855_P002	Maize	cytoskeleton, cytosol, nucleus	95.76	61.19
PGSC0003DMT400052197	Potato	cytosol	43.77	41.35
PGSC0003DMT400019210	Potato	cytosol	37.67	39.44
PGSC0003DMT400056033	Potato	cytosol	43.5	37.7
Zm00001d034421_P004	Maize	plastid	45.89	36.97
PGSC0003DMT400027956	Potato	cytosol	38.99	34.11
PGSC0003DMT400038724	Potato	cytosol	32.63	28.08

Protein Annotations

EntrezGene:102584969	MapMan:20.2.1	Gene3D:3.30.420.40	Gene3D:3.90.640.10	EMBL:AB693827	InterPro:Actin
InterPro:Actin/actin-like_CS	InterPro:Actin_CS	ProteinID:BAL60527	ProteinID:BAL60527.1	ProteinID:CAA39276	ProteinID:CAA39276.1
ProteinID:CAA39281	ProteinID:CAA39281.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005856	UniProt:M1BKU2
PFAM:PF00022	EnsemblPlantsGene:PGSC0003DMG400018449	PGSC:PGSC0003DMG400018449	EnsemblPlants:PGSC0003DMT400047481	PRINTS:PR00190	ScanProsite:PS00406
ScanProsite:PS00432	ScanProsite:PS01132	PANTHER:PTHR11937	PANTHER:PTHR11937:SF358	SMART:SM00268	SUPFAM:SSF53067
UniParc:UPI0002767FAB	EMBL:X55746	EMBL:X55752	RefSeq:XP_006355138.1	:	:

Description

Actin [Source:PGSC_GENE;Acc:PGSC0003DMG400018449]

Coordinates

chr3:+:32116228..32118781

Molecular Weight (calculated)

41760.2 Da

IEP (calculated)

5.162

GRAVY (calculated)

-0.182

Length

377 amino acids

Sequence
(BLAST)

001: MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP
101: EEHPVLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDS LMKILTERGY
201: MFTTTAEREI VRDMKEKLAY VALDYEQELE TARSSSSIEK NYELPDGQVI TIGAERFRCP EVLFQPSMIG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV
301: LSGGSTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS KGEYDESGPS IVHRKCF

Best Arabidopsis Sequence Match ( AT5G09810.1 )
(BLAST)

001: MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP
101: EEHPVLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDS LMKILTERGY
201: MFTTTAEREI VRDIKEKLAY VALDYEQELE TAKSSSSVEK NYELPDGQVI TIGAERFRCP EVLFQPSLIG MEAPGIHETT YNSIMKCDVD IRKDLYGNIV
301: LSGGSTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS KSEYDESGPS IVHRKCF

Arabidopsis Description

ACT7AtACT7 [Source:UniProtKB/TrEMBL;Acc:A0A178UEW4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.