CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

cytosol 3
plastid 5
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
EER91265
OQU91413

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G62670.1	EER91265	AT4G26630.1	25387881
AT1G62670.1	OQU91413	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d007548_P001	Maize	plastid	80.79	75.43
Zm00001d007534_P001	Maize	plastid	76.97	75.29
Zm00001d007531_P001	Maize	plastid	80.39	75.06
Zm00001d007555_P001	Maize	plastid	80.0	74.69
Zm00001d007559_P001	Maize	plastid	80.26	74.66
KXG35774	Sorghum	cytosol, mitochondrion	40.79	65.54
TraesCS1D01G058500.1	Wheat	plastid	43.82	58.42
TraesCS1D01G033100.1	Wheat	mitochondrion, plastid	59.34	57.31
TraesCS1D01G057800.1	Wheat	mitochondrion	27.24	57.02
TraesCS1D01G056600.1	Wheat	mitochondrion	15.13	56.93
TraesCS1A01G055500.1	Wheat	mitochondrion	16.18	56.68
TraesCS1B01G038400.1	Wheat	cytosol	45.26	56.58
TraesCS1A01G031600.1	Wheat	cytosol	49.21	56.5
TraesCS1B01G039700.1	Wheat	cytosol	45.0	56.25
TraesCS1B01G315500.1	Wheat	mitochondrion	23.68	56.07
TraesCS1A01G073800.1	Wheat	mitochondrion	24.47	55.86
TraesCS1A01G073700.1	Wheat	cytosol	28.29	55.84
TraesCS1D01G058000.1	Wheat	cytosol	29.74	55.66
HORVU1Hr1G010990.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	21.71	55.56
TraesCS1B01G039200.1	Wheat	mitochondrion	57.24	55.48
TraesCS1A01G067700.1	Wheat	extracellular	24.47	55.19
TraesCS1B01G038500.1	Wheat	mitochondrion	57.37	55.19
TraesCS1D01G032300.1	Wheat	mitochondrion, plastid	57.37	55.05
TraesCS1B01G039100.1	Wheat	mitochondrion	56.58	54.92
TraesCS1A01G066600.1	Wheat	mitochondrion	18.95	54.75
HORVU7Hr1G029840.1	Barley	cytosol, mitochondrion	50.26	54.65
TraesCS1D01G075800.1	Wheat	mitochondrion, plastid	24.34	54.09
TraesCS1D01G076400.1	Wheat	cytosol	25.79	53.99
TraesCS1D01G068600.1	Wheat	plastid	24.08	53.82
TraesCS1A01G067600.1	Wheat	mitochondrion	57.5	53.75
TraesCS2B01G126900.1	Wheat	cytosol	26.97	53.66
TraesCS1D01G058200.1	Wheat	extracellular, mitochondrion	49.21	53.66
HORVU1Hr1G013810.1	Barley	plastid	59.21	53.64
TraesCS1D01G056200.1	Wheat	mitochondrion	58.16	53.58
TraesCS1A01G073600.1	Wheat	mitochondrion	27.63	53.57
TraesCS1B01G085900.1	Wheat	mitochondrion	57.37	53.56
TraesCS1D01G068400.1	Wheat	mitochondrion	57.89	53.46
TraesCS1A01G031700.1	Wheat	plastid	52.11	53.44
HORVU6Hr1G017870.2	Barley	mitochondrion	21.05	53.33
TraesCS1A01G067400.1	Wheat	mitochondrion	57.89	53.33
TraesCS6D01G083300.1	Wheat	mitochondrion	31.18	53.14
HORVU1Hr1G004750.6	Barley	plastid	55.79	53.13
TraesCS1A01G054800.1	Wheat	cytosol	40.66	53.09
TraesCS1A01G054700.2	Wheat	mitochondrion	56.71	53.08
TraesCS1D01G068500.1	Wheat	mitochondrion	56.71	53.08
TraesCS1D01G068900.1	Wheat	mitochondrion	56.71	53.01
TraesCS1D01G058400.1	Wheat	cytosol	35.92	53.01
HORVU6Hr1G076780.7	Barley	mitochondrion	57.37	52.91
TraesCS6B01G127200.1	Wheat	cytosol	23.95	52.91
TraesCS1D01G068800.1	Wheat	plastid	56.71	52.82
TraesCS1B01G075300.1	Wheat	cytosol	41.45	52.76
TraesCS1D01G076800.1	Wheat	cytosol, mitochondrion	15.79	52.63
TraesCS6A01G311600.1	Wheat	mitochondrion	57.89	52.63
TraesCS1D01G033000.1	Wheat	cytosol	27.89	52.48
TraesCS1B01G038200.1	Wheat	mitochondrion	43.68	52.45
TraesCS1B01G074600.1	Wheat	mitochondrion, plastid	53.82	52.44
TraesCS1D01G076500.1	Wheat	mitochondrion	45.79	52.41
TraesCS1A01G055800.1	Wheat	mitochondrion	53.16	52.4
TraesCS6D01G290900.1	Wheat	mitochondrion	50.53	52.39
HORVU1Hr1G014040.1	Barley	mitochondrion	57.37	52.34
HORVU1Hr1G014030.2	Barley	mitochondrion, plastid	57.11	52.29
TraesCS1D01G055500.1	Wheat	cytosol, mitochondrion	46.18	52.15
TraesCS1B01G085300.1	Wheat	mitochondrion	54.08	51.89
TraesCS1B01G092500.1	Wheat	mitochondrion	53.29	51.72
HORVU1Hr1G010970.1	Barley	mitochondrion	56.18	51.69
TraesCS6B01G129200.1	Wheat	cytosol, nucleus, plastid	34.34	51.68
TraesCS1B01G038300.1	Wheat	plastid	37.11	51.65
TraesCS1B01G072300.1	Wheat	plastid	38.42	51.41
TraesCS1B01G072400.1	Wheat	cytosol	46.97	51.37
TraesCS1D01G055300.1	Wheat	cytosol	41.58	51.13
TraesCS6A01G099300.1	Wheat	cytosol, mitochondrion	32.5	51.03
TraesCS1A01G054500.1	Wheat	mitochondrion	52.24	50.9
TraesCS6A01G101100.1	Wheat	mitochondrion, plastid	33.68	50.89
TraesCS1A01G074000.1	Wheat	mitochondrion	18.95	50.88
TraesCS1B01G085400.1	Wheat	cytosol	42.89	50.86
HORVU2Hr1G105460.11	Barley	mitochondrion, plastid	32.11	50.83
HORVU1Hr1G011020.4	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	42.63	50.7
TraesCS1B01G038600.1	Wheat	cytosol	24.21	50.69
HORVU1Hr1G011400.44	Barley	plastid	41.05	50.65
TraesCS1A01G057400.1	Wheat	mitochondrion, plastid	47.76	50.63
TraesCS1A01G067000.1	Wheat	mitochondrion	47.63	50.56
TraesCS6A01G099100.1	Wheat	cytosol, mitochondrion	25.13	50.53
HORVU0Hr1G035310.1	Barley	cytosol	38.68	50.52
TraesCS6D01G083400.1	Wheat	mitochondrion	32.76	50.51
TraesCS1D01G056700.1	Wheat	cytosol	40.26	50.41
TraesCS6B01G127500.1	Wheat	cytosol	32.37	50.2
TraesCS1B01G075000.1	Wheat	cytosol, mitochondrion	43.82	50.15
TraesCS6B01G127400.1	Wheat	cytosol, mitochondrion	28.55	50.12
TraesCS1D01G068700.1	Wheat	cytosol	28.95	50.11
TraesCS6D01G089500.1	Wheat	cytosol, mitochondrion, plastid	31.71	50.1
TraesCS1A01G056000.1	Wheat	cytosol	26.45	50.0
TraesCS6D01G083100.1	Wheat	cytosol	32.24	50.0
TraesCS6B01G341800.2	Wheat	cytosol	26.18	49.87
TraesCS1B01G073200.1	Wheat	cytosol	19.87	49.84
TraesCS1D01G069100.1	Wheat	mitochondrion	41.97	49.61
TraesCS1B01G073100.1	Wheat	cytosol	39.47	49.51
HORVU0Hr1G031920.1	Barley	plastid	55.92	49.42
TraesCS1B01G086000.1	Wheat	plastid	44.61	49.42
TraesCS1A01G057300.1	Wheat	cytosol	46.45	49.16
TraesCS1B01G072900.2	Wheat	mitochondrion	56.32	49.03
TraesCS1D01G076700.1	Wheat	cytosol	21.45	48.8
TraesCS1A01G067800.1	Wheat	cytosol	23.95	48.66
TraesCS1A01G055600.1	Wheat	cytosol	20.79	48.62
TraesCS1B01G084800.1	Wheat	cytosol	29.61	48.49
TraesCS1B01G071600.1	Wheat	mitochondrion	53.95	48.35
HORVU1Hr1G011150.1	Barley	cytosol, plastid	55.13	47.45
HORVU1Hr1G014060.1	Barley	plastid	55.53	47.42
HORVU1Hr1G011160.2	Barley	cytosol, plastid	55.79	47.37
TraesCS1D01G067600.1	Wheat	cytosol	22.63	47.12
TraesCS1B01G092600.1	Wheat	plastid	31.97	47.0
TraesCS1D01G076900.1	Wheat	plastid	40.53	46.88
TraesCS1D01G057700.1	Wheat	cytosol	20.0	46.77
HORVU1Hr1G010890.5	Barley	cytosol	54.87	46.44
HORVU1Hr1G011250.1	Barley	cytosol	17.76	45.76
Os04t0350000-02	Rice	cytosol, mitochondrion, plastid	45.53	45.17
HORVU1Hr1G011300.3	Barley	plastid	54.61	45.11
TraesCS1B01G072700.1	Wheat	cytosol	30.13	44.9
OQU82832	Sorghum	cytosol	16.58	44.06
OQU82836	Sorghum	cytosol	35.26	43.79
OQU82831	Sorghum	cytosol	35.0	43.39
OQU82827	Sorghum	cytosol	45.39	43.29
OQU82823	Sorghum	cytosol	45.13	43.04
EES07882	Sorghum	cytosol	43.82	42.86
KXG27690	Sorghum	cytosol, plastid	40.79	42.41
Os04t0349600-01	Rice	plastid	21.58	42.38
KXG19780	Sorghum	cytosol	32.37	41.62
OQU80148	Sorghum	mitochondrion	39.34	41.13
EES09203	Sorghum	cytosol	43.03	40.98
Os04t0351333-00	Rice	cytosol	47.24	40.84
OQU82833	Sorghum	cytosol	23.95	40.62
OQU88594	Sorghum	cytosol	27.76	40.42
EER95949	Sorghum	mitochondrion	34.74	39.82
TraesCS6B01G129100.1	Wheat	cytosol, plastid	13.95	36.81
KXG34536	Sorghum	mitochondrion	27.5	36.54
Solyc06g007280.2.1	Tomato	cytosol	11.58	34.51
OQU82856	Sorghum	cytosol	33.55	33.91
OQU82834	Sorghum	cytosol	41.32	32.61
KRH39004	Soybean	cytosol	20.0	30.58
KRH39074	Soybean	cytosol	19.87	30.38
KRH39054	Soybean	cytosol	19.74	30.18
KRH39072	Soybean	cytosol	19.34	29.64
KRH39112	Soybean	cytosol	19.34	29.58
AT1G63330.2	Thale cress	cytosol	21.71	29.52
KRH39057	Soybean	cytosol	19.21	29.38
VIT_14s0081g00110.t01	Wine grape	cytosol	18.95	29.33
PGSC0003DMT400052977	Potato	cytosol	15.0	29.31
KRH40513	Soybean	plastid	13.42	29.06
Bra027636.1-P	Field mustard	cytosol	12.37	29.01
VIT_12s0028g02320.t01	Wine grape	mitochondrion	18.16	28.93
VIT_14s0081g00120.t01	Wine grape	plastid	21.71	28.3
Bra026882.1-P	Field mustard	cytosol	19.61	27.9
PGSC0003DMT400008759	Potato	cytosol	11.71	27.9
KRH09084	Soybean	plastid	18.68	27.79
Bra021192.1-P	Field mustard	plastid	18.42	27.78
Bra036649.1-P	Field mustard	cytosol	14.21	27.62
KRH39066	Soybean	cytosol	20.13	27.57
Bra026884.1-P	Field mustard	cytosol	16.58	27.45
Solyc06g005220.2.1	Tomato	cytosol	14.61	27.34
AT1G63400.1	Thale cress	cytosol, plastid	20.66	27.21
PGSC0003DMT400025067	Potato	cytosol	16.97	27.1
AT5G16640.1	Thale cress	mitochondrion	17.89	26.98
AT1G63080.3	Thale cress	mitochondrion	21.71	26.87
PGSC0003DMT400025087	Potato	endoplasmic reticulum	17.5	26.87
AT1G62930.1	Thale cress	plastid	22.24	26.87
KRH41817	Soybean	mitochondrion	19.61	26.85
AT1G62590.1	Thale cress	mitochondrion	22.37	26.81
KRH17148	Soybean	mitochondrion	19.21	26.79
Bra026971.1-P	Field mustard	mitochondrion	22.11	26.71
AT1G62670.1	Thale cress	mitochondrion	22.11	26.67
KRH00678	Soybean	endoplasmic reticulum, extracellular, plastid	18.42	26.67
AT1G62910.1	Thale cress	mitochondrion	22.11	26.58
VIT_14s0081g00270.t01	Wine grape	plastid	20.26	26.55
AT1G63070.1	Thale cress	mitochondrion	20.53	26.44
AT1G64580.1	Thale cress	mitochondrion	18.03	26.2
AT1G63130.1	Thale cress	mitochondrion	21.71	26.19
Bra006187.1-P	Field mustard	plastid	10.0	26.12
PGSC0003DMT400008757	Potato	cytosol	17.11	26.1
KRH48615	Soybean	mitochondrion	18.68	26.05
KRH08636	Soybean	cytosol	17.89	26.05
Bra025516.1-P	Field mustard	cytosol, mitochondrion	16.32	26.0
KRH31646	Soybean	cytosol	17.63	25.87
Bra036651.1-P	Field mustard	mitochondrion	18.16	25.79
Bra027658.1-P	Field mustard	cytosol, mitochondrion	18.42	25.78
Bra026958.1-P	Field mustard	mitochondrion	21.58	25.75
KRH08628	Soybean	plastid	18.68	25.72
AT3G22470.1	Thale cress	cytosol, mitochondrion	20.92	25.69
VIT_14s0081g00280.t01	Wine grape	mitochondrion	21.58	25.67
AT1G12300.1	Thale cress	mitochondrion	21.45	25.59
KRH08277	Soybean	plastid	19.08	25.57
Bra028267.1-P	Field mustard	mitochondrion	21.05	25.56
KRH09154	Soybean	mitochondrion	18.68	25.54
AT3G16710.1	Thale cress	mitochondrion	16.97	25.44
AT1G63150.1	Thale cress	mitochondrion	21.05	25.44
KRH08635	Soybean	cytosol, golgi, mitochondrion, plastid	16.84	25.35
AT1G62914.1	Thale cress	mitochondrion	17.5	25.19
KRH39050	Soybean	mitochondrion	17.63	25.19
AT1G62720.1	Thale cress	mitochondrion	16.05	25.15
AT1G12620.1	Thale cress	cytosol, mitochondrion	20.53	25.12
Bra027029.1-P	Field mustard	mitochondrion	8.03	25.1
Bra031731.1-P	Field mustard	mitochondrion	16.71	25.0
Solyc06g007810.1.1	Tomato	cytosol	8.82	25.0
KRH08639	Soybean	mitochondrion, plastid	18.55	24.96
AT5G41170.1	Thale cress	mitochondrion	17.24	24.86
Solyc06g007300.1.1	Tomato	extracellular	19.34	24.83
KRH25652	Soybean	cytosol, golgi	17.63	24.81
AT1G64583.1	Thale cress	mitochondrion	16.71	24.8
KRH08671	Soybean	cytosol, mitochondrion	18.03	24.77
PGSC0003DMT400053185	Potato	mitochondrion	19.21	24.75
KRH08625	Soybean	plastid	18.68	24.65
PGSC0003DMT400056665	Potato	mitochondrion	19.08	24.62
AT1G12775.1	Thale cress	cytosol, mitochondrion	20.79	24.53
KRH09121	Soybean	mitochondrion	17.24	24.53
PGSC0003DMT400025074	Potato	cytosol	17.76	24.46
KRH40400	Soybean	plastid	18.55	24.44
VIT_14s0030g02280.t01	Wine grape	plastid	18.03	24.42
AT1G62680.2	Thale cress	plastid	17.5	24.27
Bra016762.1-P	Field mustard	mitochondrion	19.34	24.22
Solyc06g007740.1.1	Tomato	cytosol	18.68	24.07
KRH08607	Soybean	cytosol	17.76	24.06
KRH09082	Soybean	cytosol	12.76	23.77
Bra026954.1-P	Field mustard	mitochondrion	20.26	23.69
AT1G12700.2	Thale cress	cytosol	19.21	23.62
AT1G06580.1	Thale cress	mitochondrion	15.53	23.6
Bra027726.1-P	Field mustard	mitochondrion	14.61	23.57
PGSC0003DMT400052971	Potato	mitochondrion	18.29	23.44
Bra026836.1-P	Field mustard	cytosol, mitochondrion	15.13	23.42
Bra026957.1-P	Field mustard	mitochondrion	18.55	23.38
Solyc04g080120.1.1	Tomato	extracellular	18.82	23.37
Bra027026.1-P	Field mustard	mitochondrion	16.05	23.33
PGSC0003DMT400053556	Potato	mitochondrion	14.08	23.31
PGSC0003DMT400056663	Potato	plastid	18.03	23.3
PGSC0003DMT400015293	Potato	mitochondrion	18.68	23.28
PGSC0003DMT400015298	Potato	mitochondrion	18.68	23.28
KRH09097	Soybean	plastid	17.76	23.16
PGSC0003DMT400008760	Potato	mitochondrion	17.63	22.87
KRH09095	Soybean	plastid	17.5	22.73
PGSC0003DMT400053182	Potato	cytosol	17.37	22.04
AT4G26800.2	Thale cress	mitochondrion, plastid	14.74	21.79
Bra026401.1-P	Field mustard	plastid	15.26	21.68
KRH08599	Soybean	cytosol, plastid	18.68	21.23
VIT_14s0030g02260.t01	Wine grape	cytosol	12.24	20.99
KRH09085	Soybean	mitochondrion	19.08	20.6
CDX68679	Canola	cytosol	3.03	20.0
OQU88581	Sorghum	cytosol	28.55	19.96
AT1G64100.2	Thale cress	cytosol	20.92	19.73
KRH12873	Soybean	cytosol	16.58	18.1
Bra027656.1-P	Field mustard	cytosol	21.45	17.32
KRH39064	Soybean	cytosol	21.32	16.72
Bra016758.1-P	Field mustard	plastid	20.0	13.11

Protein Annotations

Gene3D:1.25.40.10	MapMan:35.1	UniProt:A0A1B6PT01	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515
InterPro:IPR002885	InterPro:IPR011990	EnsemblPlants:KXG28799	ProteinID:KXG28799	ProteinID:KXG28799.1	PFAM:PF01535
PFAM:PF12854	PFAM:PF13041	PFscan:PS51257	PFscan:PS51375	PANTHER:PTHR24015	PANTHER:PTHR24015:SF874
InterPro:Pentatricopeptide_repeat	EnsemblPlantsGene:SORBI_3005G168000	SUPFAM:SSF48452	TIGRFAMs:TIGR00756	InterPro:TPR-like_helical_dom_sf	UniParc:UPI00081AC3D7
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr5:-:64657544..64660701

Molecular Weight (calculated)

83772.4 Da

IEP (calculated)

7.972

GRAVY (calculated)

0.079

Length

760 amino acids

Sequence
(BLAST)

001: MWSCARISSA VFTTAACSSP SPRAPHLALA TATARVRKGT FRPEEAHDLF DELLHQATPV PGRQLNGFLA ALARAPASAA CRDGPALAVA LFNRVSRAHG
101: PRVLSPTLHT YGILMDCCTR AHRPKLTLAF FGQVLKTGLG IDTIMISNLL RGLCEAKRTA EALDILLHRM PHLGCVPDVF SYCIVLKSLC SDRKSGQADE
201: LLRMMAEGGA VCLPNAVAYN TVIDGFFKEG DVNKACDLFN EMVQRGISPD LSTYNCVVNA LCKARAMDKA EAILRQMVDK GVLPDNWTYN SLIYGYSSTG
301: QWKEAVRVSK KMTSQGILPD VVTLNSLMAS LCKHGKIKDA RDVFDSMAMK GQKTDIFSYK IMLNGYATKG CLVDLTELFN LMLSDGIAPD SHIFNVLIKA
401: YAKCGMLDRA TIIFNEMREQ GVEPDVVTYS TVIAALCRIG KMDDAVEKFN QMIDQGVAPS ISTYHFLIQG FCTHGDLLKA KDLVLQMMNK VGKMENALRV
501: FDVMVSAGIQ PNVVVYGTLV NGYCKVGRID EGLSLFREIL HKGIKPSTTL YNIILHGLFQ AGRTVPAKVK FHEMTESGIA MDRYTYSIVL GGLFKNSCSD
601: EAILLFKELH AMNVKIDITT LNIMIAGMFQ IRRVEEAKDL FASISRSGLV PSVVTYSIMM TNLIKEGLVE EADDMFSSME NAGCEQPNSQ LLNHVVRELL
701: EKREIVRAGT YLSKIDERSF SLDHSTTTLL IDLFSSKGTC REHIRFLPAK YHFLAEASPC

Best Arabidopsis Sequence Match ( AT1G63130.1 )
(BLAST)

001: MRRLFAISST GNRFVHRSLL GKGKCGTAPP SFSHCSFWVR DFSGVRYDYR KISINRLNDL KLDDAVNLFG DMVKSRPFPS IVEFSKLLSA IAKMNKFDLV
101: ISLGEQMQNL GISHNLYTYS ILINCFCRRS QLSLALAVLA KMMKLGYEPD IVTLNSLLNG FCHGNRISDA VSLVGQMVEM GYQPDSFTFN TLIHGLFRHN
201: RASEAVALVD RMVVKGCQPD LVTYGIVVNG LCKRGDIDLA LSLLKKMEQG KIEPGVVIYN TIIDALCNYK NVNDALNLFT EMDNKGIRPN VVTYNSLIRC
301: LCNYGRWSDA SRLLSDMIER KINPNVVTFS ALIDAFVKEG KLVEAEKLYD EMIKRSIDPD IFTYSSLING FCMHDRLDEA KHMFELMISK DCFPNVVTYN
401: TLIKGFCKAK RVDEGMELFR EMSQRGLVGN TVTYTTLIHG FFQARECDNA QIVFKQMVSD GVLPDIMTYS ILLDGLCNNG KVETALVVFE YLQRSKMEPD
501: IYTYNIMIEG MCKAGKVEDG WDLFCSLSLK GVKPNVVTYT TMMSGFCRKG LKEEADALFR EMKEEGPLPD SGTYNTLIRA HLRDGDKAAS AELIREMRSC
601: RFVGDASTIG LVTNMLHDGR LDKSFLKMLS

Arabidopsis Description

Pentatricopeptide repeat-containing protein At1g63130, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q9CAN0]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.