Skip to main content
crop-pal logo
Field mustard
Search for another protein from :
Field mustard
Subcellular Localization
min:
: max

 
Winner_takes_all: cytosol

Predictor Summary:
  • cytosol 3
  • plastid 3
  • nucleus 1
  • mitochondrion 1
Predictors GFP MS/MS Papers
Winner Takes All:cytosol
Any Predictor:cytosol, mitochondrion, nucleus, plastid
BaCelLo:cytosol
iPSORT:plastid
MultiLoc:cytosol
Plant-mPloc:nucleus, plastid
PProwler:mitochondrion
WoLF PSORT:plastid
YLoc:cytosol
PPI
No PPI Data
Homology

Paralog

locusIdentityHomology Identity

Ortholog

locusHomology SpeciesLocationIdentityHomology Identity
CDY33797 Canola mitochondrion 84.08 87.7
Bra026884.1-P Field mustard cytosol 59.18 68.85
Bra026958.1-P Field mustard mitochondrion 79.59 66.72
Bra028267.1-P Field mustard mitochondrion 76.4 65.18
Bra026971.1-P Field mustard mitochondrion 75.09 63.75
Bra026836.1-P Field mustard cytosol, mitochondrion 58.24 63.34
Bra031731.1-P Field mustard mitochondrion 58.43 61.42
Bra026954.1-P Field mustard mitochondrion 74.53 61.23
Bra016762.1-P Field mustard mitochondrion 68.16 59.97
AT1G12620.1 Thale cress cytosol, mitochondrion 69.66 59.9
AT3G22470.1 Thale cress cytosol, mitochondrion 69.29 59.77
AT1G12300.1 Thale cress mitochondrion 70.6 59.18
AT1G12775.1 Thale cress cytosol, mitochondrion 66.85 55.43
Bra026957.1-P Field mustard mitochondrion 57.87 51.24
Bra027029.1-P Field mustard mitochondrion 23.22 51.03
Bra036649.1-P Field mustard cytosol 37.08 50.64
Bra027636.1-P Field mustard cytosol 29.96 49.38
Bra006187.1-P Field mustard plastid 26.03 47.77
Bra027658.1-P Field mustard cytosol, mitochondrion 46.25 45.49
Bra025516.1-P Field mustard cytosol, mitochondrion 40.45 45.28
Bra036651.1-P Field mustard mitochondrion 44.76 44.67
VIT_12s0028g02320.t01 Wine grape mitochondrion 39.7 44.44
Bra027026.1-P Field mustard mitochondrion 42.88 43.79
Bra021192.1-P Field mustard plastid 40.26 42.66
Bra027726.1-P Field mustard mitochondrion 37.08 42.04
VIT_14s0081g00270.t01 Wine grape plastid 43.07 39.66
PGSC0003DMT400008759 Potato cytosol 23.6 39.5
VIT_14s0081g00110.t01 Wine grape cytosol 36.14 39.31
Solyc06g007810.1.1 Tomato cytosol 19.29 38.43
VIT_14s0081g00120.t01 Wine grape plastid 41.2 37.74
KRH70370 Soybean cytosol 38.02 37.32
PGSC0003DMT400008757 Potato cytosol 34.46 36.95
PGSC0003DMT400052977 Potato cytosol 26.78 36.76
KRH49698 Soybean cytosol 38.39 35.96
Bra026401.1-P Field mustard plastid 35.58 35.51
KRH42744 Soybean mitochondrion 40.07 35.02
Solyc06g005220.2.1 Tomato cytosol 26.59 34.98
VIT_14s0081g00280.t01 Wine grape mitochondrion 41.76 34.9
KRH58843 Soybean nucleus 39.7 34.7
KRH75063 Soybean mitochondrion, plastid 39.14 34.6
PGSC0003DMT400025074 Potato cytosol 35.77 34.6
PGSC0003DMT400025087 Potato endoplasmic reticulum 32.02 34.55
VIT_14s0030g02280.t01 Wine grape plastid 36.14 34.4
Bra016758.1-P Field mustard plastid 74.53 34.34
KRH75048 Soybean mitochondrion 38.76 34.05
PGSC0003DMT400008760 Potato mitochondrion 37.27 33.96
KRH75045 Soybean mitochondrion 37.83 33.84
Solyc06g007300.1.1 Tomato extracellular 37.08 33.45
PGSC0003DMT400025067 Potato cytosol 29.59 33.19
KRH09256 Soybean mitochondrion 34.64 33.09
PGSC0003DMT400056665 Potato mitochondrion 36.33 32.94
PGSC0003DMT400052971 Potato mitochondrion 36.33 32.72
PGSC0003DMT400056663 Potato plastid 35.96 32.65
Solyc06g007740.1.1 Tomato cytosol 35.96 32.54
VIT_14s0030g02260.t01 Wine grape cytosol 26.97 32.51
Solyc06g007280.2.1 Tomato cytosol 15.17 31.76
KRH38755 Soybean mitochondrion 34.83 31.31
PGSC0003DMT400053185 Potato mitochondrion 34.46 31.19
PGSC0003DMT400053556 Potato mitochondrion 26.4 30.72
PGSC0003DMT400053182 Potato cytosol 33.33 29.72
TraesCS2B01G126900.1 Wheat cytosol 20.79 29.06
PGSC0003DMT400015293 Potato mitochondrion 32.4 28.36
PGSC0003DMT400015298 Potato mitochondrion 32.4 28.36
Solyc04g080120.1.1 Tomato extracellular 31.65 27.61
TraesCS1A01G067700.1 Wheat extracellular 16.85 26.71
Zm00001d018982_P001 Maize mitochondrion 17.42 26.57
HORVU1Hr1G011250.1 Barley cytosol 14.61 26.44
Bra027656.1-P Field mustard cytosol 46.25 26.25
OQU82832 Sorghum cytosol 14.04 26.22
HORVU1Hr1G010990.1 Barley cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole 14.42 25.93
Os10t0495100-01 Rice cytosol, mitochondrion 22.1 25.6
TraesCS6B01G127200.1 Wheat cytosol 16.48 25.58
Os08t0248400-01 Rice mitochondrion 20.79 25.23
TraesCS6A01G099100.1 Wheat cytosol, mitochondrion 17.79 25.13
HORVU1Hr1G018190.2 Barley cytosol 13.3 24.91
Os10t0501700-00 Rice cytosol 21.16 24.89
TraesCS6D01G083300.1 Wheat mitochondrion 20.6 24.66
TraesCS1D01G058000.1 Wheat cytosol 18.73 24.63
TraesCS6A01G015000.1 Wheat mitochondrion 19.1 24.58
TraesCS6D01G083400.1 Wheat mitochondrion 22.66 24.54
TraesCS2A01G530300.1 Wheat mitochondrion, plastid 23.41 24.46
HORVU6Hr1G017870.2 Barley mitochondrion 13.48 24.0
TraesCS6D01G089500.1 Wheat cytosol, mitochondrion, plastid 20.97 23.28
TraesCSU01G089700.1 Wheat mitochondrion 20.97 23.24
TraesCS6A01G099300.1 Wheat cytosol, mitochondrion 20.97 23.14
KXG34536 Sorghum mitochondrion 24.72 23.08
TraesCS6D01G083100.1 Wheat cytosol 21.16 23.06
Os10t0497366-00 Rice mitochondrion 17.23 22.94
OQU88594 Sorghum cytosol 22.28 22.8
TraesCS6B01G129200.1 Wheat cytosol, nucleus, plastid 21.54 22.77
TraesCS6A01G101100.1 Wheat mitochondrion, plastid 21.35 22.66
EER95949 Sorghum mitochondrion 28.09 22.62
TraesCS1D01G058400.1 Wheat cytosol 21.72 22.52
TraesCS6B01G127500.1 Wheat cytosol 20.6 22.45
TraesCSU01G090200.1 Wheat cytosol 18.54 22.4
OQU80148 Sorghum mitochondrion 30.34 22.28
Os08t0107800-01 Rice mitochondrion 26.03 22.28
TraesCS7B01G469500.1 Wheat cytosol 23.41 22.24
TraesCS1D01G069100.1 Wheat mitochondrion 26.78 22.24
KXG27690 Sorghum cytosol, plastid 29.96 21.89
OQU82856 Sorghum cytosol 30.52 21.68
Os08t0107700-01 Rice cytosol, mitochondrion 15.17 21.66
TraesCS1B01G072700.1 Wheat cytosol 20.6 21.57
Os10t0499500-01 Rice mitochondrion 20.41 21.54
TraesCS6B01G021200.1 Wheat cytosol, mitochondrion 13.11 21.54
TraesCS6A01G083000.1 Wheat cytosol 18.35 21.49
TraesCS6B01G127400.1 Wheat cytosol, mitochondrion 17.42 21.48
TraesCSU01G089800.1 Wheat mitochondrion 14.04 21.37
TraesCS6A01G014800.1 Wheat cytosol, mitochondrion 28.28 21.15
TraesCS7D01G231400.1 Wheat cytosol, plastid 15.92 21.09
EES09203 Sorghum cytosol 31.46 21.05
TraesCS1A01G031600.1 Wheat cytosol 26.03 21.0
TraesCS2B01G560000.1 Wheat mitochondrion, plastid 17.23 20.96
TraesCS1B01G038200.1 Wheat mitochondrion 24.72 20.85
TraesCS1B01G086000.1 Wheat plastid 26.78 20.85
TraesCS6B01G021600.1 Wheat mitochondrion 27.9 20.81
TraesCS6D01G076500.1 Wheat plastid 17.6 20.75
TraesCS1D01G058200.1 Wheat extracellular, mitochondrion 26.78 20.52
TraesCS1B01G075000.1 Wheat cytosol, mitochondrion 25.47 20.48
HORVU7Hr1G029840.1 Barley cytosol, mitochondrion 26.78 20.46
TraesCS1A01G031700.1 Wheat plastid 28.09 20.24
EES07882 Sorghum cytosol 29.4 20.21
OQU82823 Sorghum cytosol 30.15 20.2
Os10t0497300-01 Rice mitochondrion 29.96 20.15
TraesCS6B01G045200.1 Wheat cytosol, mitochondrion 26.97 20.14
TraesCS6D01G017800.1 Wheat cytosol 20.22 20.11
HORVU0Hr1G035310.1 Barley cytosol 21.91 20.1
OQU82836 Sorghum cytosol 23.03 20.1
HORVU6Hr1G014310.1 Barley mitochondrion 25.84 20.09
TraesCS1A01G067600.1 Wheat mitochondrion 30.52 20.05
TraesCS5B01G250800.1 Wheat cytosol, mitochondrion 26.78 20.0
TraesCS7D01G487500.1 Wheat cytosol, mitochondrion 26.78 20.0
OQU82827 Sorghum cytosol 29.78 19.95
Os10t0495200-01 Rice mitochondrion 29.21 19.95
TraesCS1B01G072400.1 Wheat cytosol 25.84 19.86
TraesCS1A01G067000.1 Wheat mitochondrion 26.59 19.83
TraesCS2D01G532400.1 Wheat cytosol 22.66 19.8
KXG19780 Sorghum cytosol 21.91 19.8
TraesCS6B01G114300.1 Wheat cytosol 14.42 19.79
TraesCS6A01G014500.1 Wheat cytosol, mitochondrion 9.36 19.76
TraesCS6A01G014600.1 Wheat cytosol 14.61 19.75
TraesCS1B01G039700.1 Wheat cytosol 22.47 19.74
TraesCS2A01G531000.1 Wheat golgi 25.09 19.73
TraesCS1B01G092600.1 Wheat plastid 19.1 19.73
TraesCS1D01G068500.1 Wheat mitochondrion 29.96 19.7
TraesCS6D01G047700.1 Wheat mitochondrion 25.47 19.68
KXG28799 Sorghum plastid 27.9 19.61
TraesCS1B01G075300.1 Wheat cytosol 21.91 19.6
TraesCS1D01G055300.1 Wheat cytosol 22.66 19.58
TraesCS2D01G533000.1 Wheat cytosol, extracellular 24.16 19.58
TraesCS1B01G085300.1 Wheat mitochondrion 29.03 19.57
TraesCS2D01G532500.1 Wheat plastid 24.91 19.56
TraesCS6D01G290900.1 Wheat mitochondrion 26.78 19.51
TraesCS1B01G085900.1 Wheat mitochondrion 29.59 19.41
TraesCS1B01G072300.1 Wheat plastid 20.6 19.37
TraesCS1A01G057300.1 Wheat cytosol 26.03 19.36
TraesCS1A01G054500.1 Wheat mitochondrion 28.28 19.36
TraesCS6D01G017700.1 Wheat plastid 19.29 19.29
TraesCS1A01G067400.1 Wheat mitochondrion 29.78 19.27
Zm00001d009733_P001 Maize cytosol 28.65 19.25
TraesCS1B01G038400.1 Wheat cytosol 21.91 19.24
KXG35774 Sorghum cytosol, mitochondrion 17.04 19.24
TraesCS2A01G526200.1 Wheat golgi 24.53 19.24
TraesCS2B01G560300.1 Wheat cytosol 20.41 19.16
TraesCS1D01G068700.1 Wheat cytosol 15.73 19.13
TraesCS1D01G068600.1 Wheat plastid 12.17 19.12
TraesCS1D01G033100.1 Wheat mitochondrion, plastid 28.09 19.06
TraesCS6B01G021500.1 Wheat cytosol, mitochondrion 13.48 19.0
TraesCS1D01G068800.1 Wheat plastid 29.03 19.0
HORVU1Hr1G014030.2 Barley mitochondrion, plastid 29.4 18.92
HORVU1Hr1G013810.1 Barley plastid 29.59 18.83
TraesCS1D01G068900.1 Wheat mitochondrion 28.65 18.82
TraesCS2B01G559900.1 Wheat cytosol 19.1 18.82
HORVU1Hr1G004750.6 Barley plastid 28.09 18.8
TraesCS1D01G056200.1 Wheat mitochondrion 29.03 18.79
HORVU2Hr1G105460.11 Barley mitochondrion, plastid 16.85 18.75
HORVU1Hr1G014040.1 Barley mitochondrion 29.21 18.73
TraesCS1B01G085400.1 Wheat cytosol 22.47 18.72
TraesCS1D01G075800.1 Wheat mitochondrion, plastid 11.98 18.71
TraesCS1D01G068400.1 Wheat mitochondrion 28.84 18.71
TraesCS1D01G032300.1 Wheat mitochondrion, plastid 27.72 18.69
TraesCS1D01G076500.1 Wheat mitochondrion 23.22 18.67
Zm00001d007555_P001 Maize plastid 28.46 18.67
TraesCS1B01G073100.1 Wheat cytosol 21.16 18.65
TraesCS1A01G054700.2 Wheat mitochondrion 28.28 18.6
TraesCS1B01G073200.1 Wheat cytosol 10.49 18.48
TraesCS2A01G531100.1 Wheat cytosol 19.66 18.45
OQU82831 Sorghum cytosol 21.16 18.43
Zm00001d007531_P001 Maize plastid 28.09 18.43
TraesCS1D01G055500.1 Wheat cytosol, mitochondrion 23.22 18.43
Zm00001d007534_P001 Maize plastid 26.78 18.4
HORVU6Hr1G076780.7 Barley mitochondrion 28.28 18.33
HORVU1Hr1G011020.4 Barley cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole 21.91 18.31
TraesCS1D01G056700.1 Wheat cytosol 20.79 18.29
TraesCS2D01G532100.1 Wheat mitochondrion 23.6 18.21
Zm00001d007548_P001 Maize plastid 27.72 18.18
Os08t0110200-01 Rice mitochondrion 27.15 18.17
TraesCS2D01G532600.1 Wheat plastid 26.4 18.17
TraesCS2A01G530600.1 Wheat cytosol 26.03 18.17
Zm00001d007559_P001 Maize plastid 27.72 18.12
HORVU6Hr1G002890.6 Barley cytosol, plastid 26.97 18.11
Os10t0497432-01 Rice mitochondrion 8.99 18.05
TraesCS1A01G054800.1 Wheat cytosol 19.66 18.04
HORVU0Hr1G031920.1 Barley plastid 28.84 17.91
TraesCS2B01G560700.1 Wheat plastid 25.84 17.9
TraesCS2D01G532300.1 Wheat plastid 26.03 17.87
TraesCS6A01G311600.1 Wheat mitochondrion 27.9 17.82
HORVU1Hr1G010970.1 Barley mitochondrion 27.34 17.68
TraesCS1A01G073700.1 Wheat cytosol 12.73 17.66
TraesCS1B01G039100.1 Wheat mitochondrion 25.84 17.62
TraesCS1B01G038500.1 Wheat mitochondrion 26.03 17.59
TraesCS1B01G074600.1 Wheat mitochondrion, plastid 25.66 17.56
HORVU1Hr1G014060.1 Barley plastid 29.21 17.53
TraesCS1B01G092500.1 Wheat mitochondrion 25.66 17.5
TraesCS1B01G084800.1 Wheat cytosol 15.17 17.46
TraesCS1A01G073800.1 Wheat mitochondrion 10.86 17.42
TraesCS1D01G076900.1 Wheat plastid 21.35 17.35
TraesCS1B01G071600.1 Wheat mitochondrion 27.53 17.33
TraesCS1B01G072900.2 Wheat mitochondrion 28.09 17.18
TraesCS1B01G315500.1 Wheat mitochondrion 10.3 17.13
TraesCS1A01G055800.1 Wheat mitochondrion 24.53 16.99
HORVU1Hr1G011160.2 Barley cytosol, plastid 28.46 16.98
TraesCS1B01G039200.1 Wheat mitochondrion 24.91 16.96
TraesCS1D01G057800.1 Wheat mitochondrion 11.42 16.8
HORVU1Hr1G011150.1 Barley cytosol, plastid 27.72 16.76
HORVU1Hr1G010890.5 Barley cytosol 28.09 16.7
TraesCS1A01G073600.1 Wheat mitochondrion 11.98 16.33
TraesCS1A01G057400.1 Wheat mitochondrion, plastid 21.91 16.32
HORVU1Hr1G011300.3 Barley plastid 28.09 16.3
Os04t0349600-01 Rice plastid 11.8 16.28
Os04t0350000-02 Rice cytosol, mitochondrion, plastid 23.22 16.19
TraesCS1A01G055500.1 Wheat mitochondrion 6.55 16.13
TraesCS6B01G341800.2 Wheat cytosol 11.98 16.04
TraesCS1D01G076700.1 Wheat cytosol 9.93 15.87
TraesCS1D01G056600.1 Wheat mitochondrion 5.99 15.84
TraesCS1D01G076800.1 Wheat cytosol, mitochondrion 6.74 15.79
TraesCS1A01G067800.1 Wheat cytosol 11.05 15.78
TraesCS1A01G056000.1 Wheat cytosol 11.8 15.67
TraesCS1D01G058500.1 Wheat plastid 16.67 15.61
Zm00001d009735_P001 Maize cytosol 28.28 15.52
OQU82833 Sorghum cytosol 12.73 15.18
OQU82834 Sorghum cytosol 27.34 15.16
TraesCS1D01G033000.1 Wheat cytosol 11.24 14.85
Os04t0351333-00 Rice cytosol 23.97 14.56
TraesCS1D01G067600.1 Wheat cytosol 9.93 14.52
TraesCS1B01G038300.1 Wheat plastid 14.79 14.47
TraesCS1A01G074000.1 Wheat mitochondrion 7.49 14.13
TraesCS1B01G038600.1 Wheat cytosol 9.55 14.05
TraesCS1A01G066600.1 Wheat mitochondrion 6.74 13.69
TraesCS1D01G076400.1 Wheat cytosol 8.99 13.22
HORVU1Hr1G011400.44 Barley plastid 14.98 12.99
TraesCS1A01G055600.1 Wheat cytosol 7.49 12.31
TraesCS6B01G129100.1 Wheat cytosol, plastid 6.55 12.15
TraesCS1D01G057700.1 Wheat cytosol 7.3 12.0
OQU88581 Sorghum cytosol 21.72 10.67
TraesCS2D01G532200.1 Wheat cytosol 5.43 9.9
Protein Annotations
EnsemblPlants:Bra026882.1EnsemblPlants:Bra026882.1-PEnsemblPlantsGene:Bra026882Gene3D:1.25.40.10GO:GO:0003674GO:GO:0005488
GO:GO:0005515InterPro:IPR002885InterPro:IPR011990InterPro:Pentatricopeptide_repeatInterPro:TPR-like_helical_dom_sfPANTHER:PTHR44149
PANTHER:PTHR44149:SF1PFAM:PF12854PFAM:PF13041PFscan:PS51375SUPFAM:SSF81901TIGRFAMs:TIGR00756
UniParc:UPI0002542E8FUniProt:M4EDM2MapMan:35.1:::
Description
AT1G12700 (E=2e-189) | helicase domain-containing protein / pentatricopeptide (PPR) repeat-containing protein
Coordinates
chrA09:+:34089765..34091469
Molecular Weight (calculated)
59166.4 Da
IEP (calculated)
4.909
GRAVY (calculated)
0.080
Length
534 amino acids
Sequence
(BLAST)
001: MIQSRPLPTV VDFSRLFSGI ARTKQYDLVL SLCKQMESNG IAHDLYTLSI VINCFCRRRK LGFAFSVFGK MLKLGYEPTT VTFSTLINGL CLEGRVSEAV
101: ELVDRMVEMK VVPNLIILNT LVNGLCLQDR VSEAVALIDR MMSTRSIYLW SGFEQNVLCK DESLDDALSL FSEMETKGIK PNVFTYTSLI GGFCNAGRWD
201: DGAQLLRDMI TRDITPDVVT FNALIDSFVK EGKLTEAQEL YNEMITRGID PNTITYTTLI YGLCMENRLD EANQMMDLMV SKGCDPNSVT YSILINGYCK
301: NKLVDEGMRL FRKMSLRGVV ADTVTYSTLI QGFCQTGKPK VAKDLFQEMV SIGVPPSVVT YGILLDGLCD NGELEKALEI LDQMHNCKMD PGICIYSIII
401: HGMCNAGKVD GAWDLFCSLP SKGVKPNVKT FTVMISGLCK KGSLPEANIL LRKMEEDGIV PNVCTYNTLI RAHLSGGDIS NSVELIEEMK RCGFSADAST
501: IKMVMDMLSD GRLDKSFLDM LSGPFGDKSS SSLD
Best Arabidopsis Sequence Match ( AT1G12300.1 )
(BLAST)
001: MVKLMIRRLS SQVSKFVQPR LLETGTLRIA LINCPNELSF CCERGFSAFS DRNLSYRERL RSGLVDIKAD DAIDLFRDMI HSRPLPTVID FSRLFSAIAK
101: TKQYDLVLAL CKQMELKGIA HNLYTLSIMI NCFCRCRKLC LAFSAMGKII KLGYEPNTIT FSTLINGLCL EGRVSEALEL VDRMVEMGHK PDLITINTLV
201: NGLCLSGKEA EAMLLIDKMV EYGCQPNAVT YGPVLNVMCK SGQTALAMEL LRKMEERNIK LDAVKYSIII DGLCKHGSLD NAFNLFNEME MKGITTNIIT
301: YNILIGGFCN AGRWDDGAKL LRDMIKRKIN PNVVTFSVLI DSFVKEGKLR EAEELHKEMI HRGIAPDTIT YTSLIDGFCK ENHLDKANQM VDLMVSKGCD
401: PNIRTFNILI NGYCKANRID DGLELFRKMS LRGVVADTVT YNTLIQGFCE LGKLNVAKEL FQEMVSRKVP PNIVTYKILL DGLCDNGESE KALEIFEKIE
501: KSKMELDIGI YNIIIHGMCN ASKVDDAWDL FCSLPLKGVK PGVKTYNIMI GGLCKKGPLS EAELLFRKME EDGHAPDGWT YNILIRAHLG DGDATKSVKL
601: IEELKRCGFS VDASTIKMVI DMLSDGRLKK SFLDMLS
Arabidopsis Description
Pentatricopeptide repeat-containing protein At1g12300, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q0WKV3]
SUBAcon: [mitochondrion]
Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.